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The penetration features of the hydrofobized hyaluronic acid – based polymeric micelles.
Mischingerová, Monika ; Pekař, Miloslav (referee) ; Muthný, Tomáš (advisor)
The aim of this thesis was to investigate the penetration features of the hydrofobized hyaluronic acid – based polymeric micelles using Nile red as carried tracer. Furthermore, to implement basic characterization of polymeric micelles for potential cosmetic applications using Coenzyme Q10 (CoQ10) as carried substance. It was found that the size of the polymeric micelles with carried CoQ10 did not exceed 100 nm. Applied delivery systems based on hydrophobic hyaluronic acid were suitable for potential topical application. Delivery systems with Nile Red as carried tracer demonstrated excellent penetration features. We assume that delivery systems with CoQ10 will exhibit similar penetration features. An issue has appeared whether the carrier breaks or proceeds along with NR to the skin. Moreover, another experiments have been designed which could also verify the penetration features of these systems.
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Study of aggregation process and physical stability of polymeric micelle by fluorescence probe method
Chovancová, Romana ; Pekař, Miloslav (referee) ; Mravec, Filip (advisor)
Amphiphilic block copolymer poly(ethylene glycol)-b-poly(–caprolactone) (PEG–PCL) was synthesized from poly(ethylene glycol) (PEG) and poly(–caprolactone) (PCL) by using ring-opening polymerization. The structure and composition of this copolymer was determined by IR spectroscopy. Polymeric micelles were prepared by membrane dialysis method and direct dissolution of copolymer in water. The process of aggregation and physical stability in water solution was studied by fluorescence spectroscopy using pyrene and perylene as fluorescent probes.The results of steady-state and time resolved fluorescence measurements indicate that system of PEG–PCL forms both unimolecular and multimolecular micelles, which depends on copolymer concentration. The critical micelle concentration (CMC) was around 0,002 g/L. Measurement of micelles size by dynamic light scattering method suggested that systems with higher concentration form bigger aggregates. In addition, copolymer behavior was explored under physiological conditions. The results show that CMC of copolymer increased in 0,15 mol/L sal solution at temperature 37°C to 0,02 g/L when compared to copolymer in water.
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The penetration features of the hydrofobized hyaluronic acid – based polymeric micelles.
Mischingerová, Monika ; Pekař, Miloslav (referee) ; Muthný, Tomáš (advisor)
The aim of this thesis was to investigate the penetration features of the hydrofobized hyaluronic acid – based polymeric micelles using Nile red as carried tracer. Furthermore, to implement basic characterization of polymeric micelles for potential cosmetic applications using Coenzyme Q10 (CoQ10) as carried substance. It was found that the size of the polymeric micelles with carried CoQ10 did not exceed 100 nm. Applied delivery systems based on hydrophobic hyaluronic acid were suitable for potential topical application. Delivery systems with Nile Red as carried tracer demonstrated excellent penetration features. We assume that delivery systems with CoQ10 will exhibit similar penetration features. An issue has appeared whether the carrier breaks or proceeds along with NR to the skin. Moreover, another experiments have been designed which could also verify the penetration features of these systems.
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Study of aggregation process and physical stability of polymeric micelle by fluorescence probe method
Chovancová, Romana ; Pekař, Miloslav (referee) ; Mravec, Filip (advisor)
Amphiphilic block copolymer poly(ethylene glycol)-b-poly(–caprolactone) (PEG–PCL) was synthesized from poly(ethylene glycol) (PEG) and poly(–caprolactone) (PCL) by using ring-opening polymerization. The structure and composition of this copolymer was determined by IR spectroscopy. Polymeric micelles were prepared by membrane dialysis method and direct dissolution of copolymer in water. The process of aggregation and physical stability in water solution was studied by fluorescence spectroscopy using pyrene and perylene as fluorescent probes.The results of steady-state and time resolved fluorescence measurements indicate that system of PEG–PCL forms both unimolecular and multimolecular micelles, which depends on copolymer concentration. The critical micelle concentration (CMC) was around 0,002 g/L. Measurement of micelles size by dynamic light scattering method suggested that systems with higher concentration form bigger aggregates. In addition, copolymer behavior was explored under physiological conditions. The results show that CMC of copolymer increased in 0,15 mol/L sal solution at temperature 37°C to 0,02 g/L when compared to copolymer in water.
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